Featured Joint Publications

Bioinformatics & Physics

Reliability of genomic variants across different next-generation sequencing platforms and bioinformatic processing pipelines. S, Weißbach, S. Sys, C. Hewel1, H. Todorov, S. Schweiger, J. Winter, M. Pfenninger, A. Torkamani, D. Evans, J. Burger, K. Everschor-Sitte, H. May-Simera, and S. Gerber. BMC Genomics 22, 62 (2021).
https://doi.org/10.1186/s12864-020-07362-8

Next Generation Sequencing (NGS) is the fundament of various studies, providing insights into questions from biology and medicine. Nevertheless, integrating data from different experimental backgrounds can introduce strong biases. In order to methodically investigate the magnitude of systematic errors in single nucleotide variant calls, we performed a cross-sectional observational study on a genomic cohort of 99 subjects each sequenced via (i) Illumina HiSeq X, (ii) Illumina HiSeq, and (iii) Complete Genomics and processed with the respective bioinformatic pipeline. We also repeated variant calling for the Illumina cohorts with GATK, which allowed us to investigate the effect of the bioinformatics analysis strategy separately from the sequencing platform's impact. We provide empirical evidence of systematic heterogeneity in variant calls between alternative experimental and data analysis setups. Furthermore, our results demonstrate the benefit of reprocessing genomic data with harmonized pipelines when integrating data from different studies.

Geological Sciences & Mathematics

Inferring rheology and geometry of subsurface structures by adjoint-based inversion of principal stress directions. G.S. Reuber, L. Holbach, A.A. Popov, M. Hanke, and B.J.P. Kaus. Geophys. J. International 223.2 (2020): 851-861.

https://doi.org/10.1093/gji/ggaa344

Imaging subsurface structures, such as salt domes, magma reservoirs or subducting plates, is a major challenge in geophysics. Seismic imaging methods are, so far, the most precise methods to open a window into the Earth. However, the methods may not yield the exact depth or size of the imaged feature and may become distorted by phenomena such as seismic anisotropy, fluid flow, or compositional variations. A useful complementary method is therefore to simulate the mechanical behaviour of rocks on large timescales, and compare model predictions with observations. Recent studies have used the (non-linear) Stokes equations and geometries from seismic studies in combination with an adjoint-based approach to invert for rheological parameters that are consistent with surface observations such as GPS velocities. Nevertheless, it would be useful to use other surface observations, such as principal stress directions, as constraints as well. Here, we derive the adjoint formulation for the case that principal stress directions are used as observables with respect to rheological parameters. Both an algebraic and a discretized derivation of the adjoint equations are described. This thus enables the usage of two data fields - surface velocities and stress directions - as a misfit for the inversion. We test the performance of the inversion for principal stress directions on simplified 3-D test cases.

Finally, we demonstrate how the adjoint approach can be used to compute 3-D geodynamic sensitivity kernels, which highlight the areas in the model domain that have the largest impact on the misfit value of a particular point. This provides a simple, yet powerful, way to visualize which parts of the model domain are of key importance if changing rheological constants.